pEGFP
pEGFP
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pEGFP is a high-copy reporter and cloning vector featuring an IPTG-inducible lac promoter driving expression of enhanced green fluorescent protein (EGFP) in E. coli. It carries an Ampicillin resistance marker (AmpR) and uses a pUC origin of replication (ColE1-derived), supporting a high plasmid copy number. A multiple cloning site (MCS) located between the lac promoter and EGFP coding sequence allows flexible insertion of target genes, enabling visual screening of recombinants. Ideal for applications requiring a built-in positive control and convenient antibiotic selection.
Applications: Cloning
Expression: Inducible
Gene: EGFP
Codon Optimization: Not applicable
Host: E. coli
Selection markers: Ampicilin / Carbenicillin (E. coli)
Copy number: High-copy number
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pEGFP Plasmid
pEGFP is a high-copy, IPTG-inducible reporter and cloning plasmid designed for robust expression of enhanced green fluorescent protein (EGFP) in E. coli. The plasmid utilizes a lac promoter/operator system, enabling controlled induction of EGFP expression with IPTG, while the upstream CAP binding site supports catabolite repression for tighter regulation. A strategically placed multiple cloning site (MCS) upstream of the EGFP coding region allows insertion of foreign genes, disrupting green fluorescence and providing an effective screen for recombinant clones. The pUC origin of replication (ColE1-derived) supports a high plasmid copy number (~500 copies per cell), and the Ampicillin resistance marker ensures straightforward antibiotic selection.
pEGFP is frequently used in bacterial cloning workflows where a built-in fluorescent positive control is desired, providing a simple way to verify transformation efficiency while supporting standard molecular biology protocols in E. coli.
GenBank Accession: U76561.1.
Format, Storage, Shipping
- Concentration: 500 ng/µL
- Buffer: 10 mM Tris-HCl, 1 mM EDTA (TE buffer), pH 8.0
- Form: Liquid, ready to use
- Storage: –20 °C
- Shipping: UPS Next Day Air