GenoCAD
Plasmid Fragment - Cloning Grade
Plasmid Fragment - Cloning Grade
Streamline your cloning workflows with ready-to-use linear DNA fragments.
Our Cloning Grade plasmid fragments save you time and reduce risk by delivering high-quality, DpnI-treated PCR products. These blunt-ended fragments are ideal for Gibson Assembly and other homology-based cloning methods. When needed, PCR fragments can be digested with restriction enzymes to generate cohesive ends compatible with traditional cloning vectors or modular assembly standards such as Golden Gate and MoClo.
Plasmid fragments are available in three scales to support a wide range of applications—from routine cloning to complex assemblies and high-throughput library construction.
For applications such as IVT or cell-free protein synthesis that require large quantities of linearized plasmid DNA, please refer to our Plasmid Linearization service.
Volume Discounts:
- 10% for orders of 24 plasmids.
- 20% for orders of 96 plasmids.
Couldn't load pickup availability
Share
Fragment Production Process
Plasmid fragments are generated using high-fidelity PCR amplification with primers designed to amplify the expression cassette, a specific plasmid component, or the entire plasmid. Following amplification, the PCR product is treated with DpnI to selectively degrade the methylated plasmid template, ensuring that only newly synthesized linear DNA remains. The resulting fragments are then purified to remove enzymes, primers, and free nucleotides, and eluted in Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 8.0).
This streamlined process yields high-purity, cloning-ready linear DNA, suitable for downstream applications such as Gibson Assembly, HiFi Assembly, or restriction enzyme-based ligation workflows. Optional restriction digestion can be performed upon request to generate cohesive ends compatible with traditional cloning vectors or modular cloning systems like Golden Gate or MoClo.
Choosing the Right Scale for Your Application
Cloning Scale (~10 µg) Ideal for targeted cloning projects that depend on hard-to-obtain DNA fragments. This scale supports labs that prefer to perform their own plasmid assembly but need help generating challenging or high-value fragments not easily sourced or assembled in-house.
Assembly Scale (~25 µg) Designed for medium-scale, high-throughput plasmid assembly workflows where the same fragment may be used in multiple constructs. This format provides sufficient yield for parallel cloning or large batch builds without the need for repeated PCRs.
Library Scale (~100 µg) Optimized for large-scale applications such as antibody library construction or screening campaigns involving dozens to hundreds of constructs. These pooled, batch-purified fragments are ready for high-efficiency downstream assembly or cell-based screening.
Each scale is PCR-based, DpnI-treated, and purified to deliver consistent, high-integrity linear DNA, reducing the risk of contamination and saving valuable lab time.
Quality Control
All plasmid fragment preparations undergo a three-point quality control process to ensure suitability for demanding research and preclinical applications:
- UV absorbance at 230/260/280 nm to assess concentration and purity
- NGS-based sequencing to verify plasmid identity and integrity
- Capillary electrophoresis to confirm that >95% of the product is fully linear
Final preparations are delivered in Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 8.0) at a standard concentration of 500 ng/µL. Alternative delivery formats—such as nuclease-free water or lyophilized DNA—are available upon request.