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CV-CLOBSII (Eq. pBluescript-II-SK(-))

CV-CLOBSII (Eq. pBluescript-II-SK(-))

$99.00 USD
$99.00 USD
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CV-CLOBSII has a sequence identical to the pBluescript II SK(–) cloning vector. This high-copy plasmid carries an ampicillin resistance marker and a ColE1-derived origin, and features an extended multiple cloning site within the lacZα fragment for blue–white screening. With dual T3 and T7 promoter sites flanking the MCS, it is widely used for cloning, sequencing, and in vitro transcription applications in E. coli.

Format: 2 tubes with 5 µg in 5 µl of TE

Key Properties

Applications: Cloning

Copy number: High-copy number

Host: E. coli

Selection markers: Amp / Carb (E. coli)

Sequence: CV-CLOBSII_Eq._pBluescript-II-SK.gb

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Background

pBluescript II SK(–) was developed as a versatile cloning and sequencing vector, incorporating features for blue–white screening, bacteriophage promoter-driven transcription, and high-copy replication in E. coli. Its design made it one of the most widely used vectors in molecular biology for DNA manipulation and in vitro transcription. CV-CLOBSII preserves the exact sequence of pBluescript II SK(–), ensuring compatibility with published protocols and sequence data. The original description can be found in: Short, J.M., Fernandez, J.M., Sorge, J.A., & Huse, W.D. (1988). λZAP: A bacteriophage λ expression vector with in vivo excision properties. Nucleic Acids Res., 16(15), 7583–7600.

Features

  • Antibiotic selection: Ampicillin (bla)
  • Origin of replication: ColE1-derived, high copy (~500–700/cell)
  • Size: ~2.96 kb
  • MCS: Expanded multiple cloning site within the lacZα fragment for blue–white screening
  • Promoter elements: Flanking T3 and T7 promoters for in vitro transcription and sequencing applications
  • Orientation: SK(–) configuration

Applications

  • Routine cloning and subcloning in E. coli
  • Blue–white screening of recombinant colonies
  • DNA sequencing template preparation
  • In vitro transcription from T3 or T7 promoters
  • Versatile backbone for molecular biology method development

Limitations

  • Single selection marker (ampicillin)
  • High copy number may impose metabolic burden on host cells
  • Primarily designed for cloning, not protein expression
  • SK(–) orientation may require consideration when using specific promoter–insert combinations