1 1

CV-COLCDF (Eq. pCDFDuet-1)

CV-COLCDF (Eq. pCDFDuet-1)

$149.00 USD
$149.00 USD
Sale Sold out
Shipping calculated at checkout.

CV-COLCDF has a sequence identical to the pCDFDuet-1 co-expression vector. This plasmid carries a streptomycin/spectinomycin resistance marker and a CloDF13 origin of replication, ensuring stable maintenance at low copy number. It includes two T7 promoter-driven multiple cloning sites with ribosome binding sites, enabling coordinated expression of two target genes in E. coli.

Format: 2 tubes with 5 µg in 5 µl of TE

Key Properties

Applications: Bacterial Expression

Copy number: Low copy number

Expression: Constitutive

Host: E. coli

Selection markers: Streptomycin (E. coli)

Sequence: CV-COLCDF_Eq._pCDFDuet-1.gb

View full details

Background

pCDFDuet-1 was introduced by Novagen as part of the Duet™ vector family, designed to enable co-expression of two target genes in E. coli. CV-COLCDF preserves the exact pCDFDuet-1 sequence, ensuring compatibility with published protocols and literature. The CloDF13 origin confers a low copy number, making this plasmid particularly useful for expressing proteins that may be toxic when overproduced. Original documentation is available in the Novagen manuals: pCDFDuet-1 Vector Manual (PDF) and Duet Vectors Protocol TB340 (PDF).

Features

  • Antibiotic selection: Streptomycin/spectinomycin (aadA)
  • Origin of replication: CloDF13 (low copy, ~5–10/cell)
  • Size: ~4.3 kb
  • Expression system: Two T7 promoters, each with a ribosome binding site and multiple cloning site
  • Compatibility: Low-copy origin allows stable co-maintenance with ColE1- and p15A-derived vectors in the same cell.

Applications

  • Co-expression of proteins that are toxic at high copy number
  • Balanced multi-protein complex assembly
  • Metabolic engineering where lower plasmid load improves host viability
  • Protein–protein interaction assays
  • Combination with other Duet vectors for multi-plasmid systems

Limitations

  • Requires a host strain expressing T7 RNA polymerase (e.g., BL21(DE3))
  • Lower plasmid yields compared to medium- or high-copy vectors
  • No regulatory system beyond the T7 promoter; risk of basal expression
  • Restricted to E. coli expression