pBAD24
pBAD24
SKU:CL-BAD24-50
pBAD24 is a medium-copy expression vector featuring the arabinose-inducible PBAD promoter for tightly regulated gene expression in E. coli. It carries an Ampicillin resistance marker (AmpR) and uses a ColE1 origin of replication, resulting in higher plasmid copy number than pBAD18. Ideal for applications requiring stronger expression while maintaining inducibility.
Applications: Cloning
Expression: Inducible
Gene: Not applicable
Codon Optimization: Not applicable
Host: E. coli
Selection markers: Ampicilin / Carbenicillin (E. coli)
Copy number: Medium copy number
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pBAD24 Plasmid
pBAD24 is a medium-copy, arabinose-inducible expression plasmid designed for tightly controlled gene expression in Escherichia coli. It utilizes the PBAD promoter, regulated by the AraC protein, allowing tunable gene expression in response to varying concentrations of L-arabinose. This system provides low background expression and strong induction, making it ideal for expressing proteins that are toxic, require controlled folding, or interfere with host metabolism.
pBAD24 carries an Ampicillin resistance marker (AmpR) for antibiotic selection and a ColE1 origin of replication, supporting a moderate to high plasmid copy number (~100–200 copies per cell). Compared to pBAD18 (which uses a p15A origin), pBAD24 enables higher protein yields while preserving inducibility.
pBAD24 is frequently used in bacterial expression workflows requiring precise control over protein production and is compatible with standard cloning and transformation protocols.
GenBank Accession: X81837.1.
Format, Storage, Shipping
- Concentration: 500 ng/µL
- Buffer: 10 mM Tris-HCl, 1 mM EDTA (TE buffer), pH 8.0
- Form: Liquid, ready to use
- Storage: –20 °C
- Shipping: UPS Next Day Air