CV-CLO322 (Eq. pBR322)
CV-CLO322 (Eq. pBR322)
CV-CLO322 has a sequence identical to the historic pBR322 cloning vector. It carries both ampicillin and tetracycline resistance markers and a low–to–medium copy ColE1 origin. This plasmid is suitable as a control or as a versatile cloning backbone in E. coli.
Format: 2 tubes with 5 µg in 5 µl of TE
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Key Properties
Applications: Cloning
Copy number: Low copy number
Host: E. coli
Selection markers: Amp / Carb (E. coli) and Tetracycline (E. coli)
Sequence: CV-CLO322_Eq._pBR322.gb
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Background
First described in 1977 (Bolivar et al., Gene 2:95–113), pBR322 became a cornerstone of molecular cloning. CV-CLO322 preserves the original sequence, ensuring compatibility with the extensive literature and established protocols.
Features
- Antibiotic selection: Ampicillin (bla) and tetracycline (tetA)
- Origin of replication: ColE1 (low–to–medium copy, ~15–20/cell)
- Size: 4.36 kb (compact and easy to manipulate)
Applications
- Transformation control
- Electrophoresis marker
- Routine cloning and subcloning in E. coli
- Educational labs and training modules
- Backbone for constructing derivative vectors
Limitations
- Lower copy number than pUC-derived vectors → reduced plasmid yield
- No expression cassette for protein production
- Outdated design compared to modern vectors
- Limited sequence annotation in the original literature