1 2

pBR322

pBR322

$99.00 USD
$99.00 USD
Sale Sold out
Shipping calculated at checkout.

CL-BR322-50

Quantity

pBR322 is one of the first plasmid vectors used in molecular biology. Developed in 1977, it features antibiotic resistance genes for ampicillin and tetracycline, a moderate-copy origin of replication, and multiple unique restriction sites, making it a foundational tool for cloning and gene expression in E. coli.

Applications: Cloning

Expression: Not applicable

Gene: Not applicable

Codon Optimization: Not applicable

Host: E. coli

Selection markers: Ampicilin / Carbenicillin (E. coli) and Tetracyclin (E. coli)

Copy number: Medium copy number
View full details

pBR322 Plasmid

pBR322 is one of the most iconic and historically significant plasmid vectors in molecular biology. Originally developed in 1977 by Bolivar and Rodriguez, it set the foundation for modern cloning and remains a valuable reference tool in research and education.

This 4,361 bp plasmid includes:

  • AmpR – β-lactamase gene conferring resistance to ampicillin
  • TetR – tetracycline resistance gene
  • ColE1 origin of replication – medium copy number replication origin
  • rop – regulator of plasmid replication, helping to maintain stability

The vector is ideal for:

  • Benchmarking experiments
  • Teaching and historical comparison
  • Use as a backbone in new construct designs
  • Confirming transformation protocols or antibiotic selection

GenBank Accession: J01749.1

Format, Storage, Shipping

  • Concentration: 500 ng/µL
  • Buffer: 10 mM Tris-HCl, 1 mM EDTA (TE buffer), pH 8.0
  • Form: Liquid, ready to use
  • Storage: –20 °C
  • Shipping: UPS Next Day Air