CV-CLO19 (Eq. pUC19)
CV-CLO19 (Eq. pUC19)
CV-CLO19 sequence is identical to that of the classic pUC19 cloning vector. This high-copy plasmid carries an ampicillin resistance marker and a ColE1-derived origin, and features a multiple cloning site within the lacZα gene for blue–white screening in E. coli.
Format: 2 tubes with 5 µg in 5 µl of TE
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Key Properties
Applications: Cloning
Copy number: High-copy number
Host: E. coli
Selection markers: Amp / Carb (E. coli)
Sequence: CV-CLO19_Eq._pUC19.gb
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Background
Developed in the early 1980s, pUC19 became one of the most widely used E. coli cloning vectors. CV-CLO19 preserves the exact pUC19 sequence, making it fully compatible with established protocols and widely cited literature. Its high copy number and blue–white screening capability made it a workhorse for molecular cloning.
Features
- Antibiotic selection: Ampicillin (bla)
- Origin of replication: ColE1-derived, high copy (~500–700 copies/cell)
- Size: 2.7 kb (small and easy to manipulate)
- MCS: Multiple cloning site inserted within lacZα fragment for blue–white screening
Applications
- Routine cloning and subcloning in E. coli
- Blue–white screening of recombinant colonies
- High-yield plasmid DNA preparation
- Backbone for derivative vector construction
- Educational labs and training exercises
Limitations
- Only a single selection marker (ampicillin)
- Not suitable for protein expression without additional elements
- Higher metabolic burden on host cells compared to lower-copy plasmids