pUC19
pUC19
SKU:CL-UC19-50
pUC19 is a classic high-copy plasmid vector widely used for general cloning and subcloning in E. coli. It features a multiple cloning site (MCS) within the lacZα gene for blue/white screening, an Ampicillin resistance marker, and a ColE1-derived origin that supports exceptionally high plasmid yields. Its reliability and simplicity have made it a staple in molecular biology for decades.
Applications: Cloning
Expression: Not applicable
Gene: Not applicable
Codon Optimization: Not applicable
Host: E. coli
Selection markers: Ampicilin / Carbenicillin (E. coli)
Copy number: High-copy number
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pUC19 Plasmid
pUC19 is a high-copy plasmid vector designed for efficient DNA cloning and propagation in Escherichia coli. It contains a versatile multiple cloning site (MCS) embedded within the lacZα gene, enabling blue/white screening of recombinants through α-complementation. This feature makes pUC19 particularly useful in workflows that involve screening for successful ligation events.
pUC19 carries an Ampicillin resistance marker (AmpR) for antibiotic selection and replicates using a ColE1-derived origin of replication optimized for very high copy number (~500–700 copies per cell). The high yield of plasmid DNA makes it ideal for insert propagation, subcloning, and use as a backbone for custom vector construction.
pUC19 is nearly identical to pUC18, differing only in the orientation of the MCS, which can facilitate specific directional cloning strategies. Its simplicity, reliability, and high plasmid yield have made it a staple in both research and teaching laboratories.
GenBank Accession: M77789.2
Format, Storage, Shipping
- Concentration: 500 ng/µL
- Buffer: 10 mM Tris-HCl, 1 mM EDTA (TE buffer), pH 8.0
- Form: Liquid, ready to use
- Storage: –20 °C
- Shipping: UPS Next Day Air