pUC57
pUC57
SKU:CL-UC57-50
pUC57 is a high-copy cloning vector derived from pUC19 and designed for Exonuclease III (ExoIII) deletion mapping. It features a compact multiple cloning site (MCS) with six restriction sites that produce 3′ overhangs resistant to ExoIII digestion. With a ColE1 origin and AmpR marker, pUC57 is ideal for generating unidirectional deletions or as a compact general-purpose cloning backbone.
Applications: Cloning
Expression: Not applicable
Gene: Not applicable
Codon Optimization: Not applicable
Host: E. coli
Selection markers: Ampicilin / Carbenicillin (E. coli)
Copy number: High-copy number
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pUC57 Plasmid
pUC57 is a compact, high-copy plasmid vector derived from pUC19, specifically engineered for Exonuclease III (ExoIII) deletion mapping and general cloning applications in Escherichia coli. It features a streamlined multiple cloning site (MCS) with six unique restriction sites (EcoRI, SacI, KpnI, SmaI, BamHI, XbaI) that produce 3′ overhangs resistant to ExoIII digestion. This allows researchers to perform unidirectional deletion studies with high precision and control.
The plasmid includes a β-lactamase gene (AmpR) for ampicillin resistance and a ColE1-derived origin of replication, supporting very high plasmid copy numbers (~500–700 copies per cell). Its compact size (2710 bp) facilitates efficient transformation and high-yield plasmid production.
While lacking the extended MCS and promoter elements found in larger synthetic biology vectors, pUC57 remains a reliable choice for cloning, insert storage, and deletion analysis. It retains the lacZα fragment for potential blue/white screening, though it does not include a lac promoter/operator or transcriptional control elements.
GenBank Accession: Y14837.1
Format, Storage, Shipping
- Concentration: 500 ng/µL
- Buffer: 10 mM Tris-HCl, 1 mM EDTA (TE buffer), pH 8.0
- Form: Liquid, ready to use
- Storage: –20 °C
- Shipping: UPS Next Day Air