1 1

CV-CLO57 (Eq. pUC57)

CV-CLO57 (Eq. pUC57)

$99.00 USD
$99.00 USD
Sale Sold out
Shipping calculated at checkout.

CV-CLO57 has a sequence identical to the classic pUC57 cloning vector. This high-copy plasmid carries an ampicillin resistance marker and a ColE1-derived origin, and includes a multiple cloning site within the lacZα fragment for blue–white screening in E. coli. Its compact size makes it a versatile backbone for general cloning applications.

Format: 2 tubes with 5 µg in 5 µl of TE

Key Properties

Applications: Cloning

Copy number: High-copy number

Host: E. coli

Selection markers: Amp / Carb (E. coli)

Sequence: CV-CLO57_Eq._pUC57.gb

View full details

Background

pUC57 is a synthetic derivative of the pUC series, widely used as a general-purpose cloning vector. It retains the key features of pUC19, including the high-copy ColE1-derived origin and lacZα multiple cloning site, while providing a streamlined backbone optimized for custom cloning. CV-CLO57 preserves the exact sequence of pUC57 as referenced in GenBank: Y14837.1, ensuring full compatibility with published protocols and sequence data.


Features

  • Antibiotic selection: Ampicillin (bla)
  • Origin of replication: ColE1-derived, high copy (~500–700/cell)
  • Size: ~2.7 kb
  • MCS: Multiple cloning site within lacZα fragment for blue–white screening
  • Design: Compact synthetic backbone with minimal nonessential sequence


Applications

  • Routine cloning and subcloning in E. coli
  • Blue–white screening of recombinant colonies
  • High-yield plasmid DNA preparation
  • Template backbone for custom constructs
  • Educational and teaching applications


Limitations

  • Single selection marker (ampicillin) limits flexibility
  • Not designed for protein expression without added regulatory elements
  • High copy number may place a metabolic burden on the host in some contexts